Synthesis and Identification of Novel 1,3-Oxazepane-7,4-Dione Compounds via the Reaction of Succinic Anhydride with Schiff's Base and Assessment of Biological Activity

This research showed how to use a cycloaddition method to make and describe new cyclic compounds, mainly oxazepine derivatives. Terephthalaldehyde and substituted aniline reacted with each other in an ethanol solvent under acidic conditions. Glacial acetic acid was used as a catalyst in this reaction. The final product was 1,1'-(1,4-phenylene)bis(N-substituted-methenamine) (H1-H6). We subsequently subjected these azomethines to pericyclic production in dry benzene using succinic anhydride, resulting in the production of 2,2'-(1,4-phenylene)bis(3-(substituted)-1,3-oxazepane-4,7-dione) (H7-H12). We employed the FT-IR, 1H-NMR, and 13C-NMR spectra to investigate the physicochemical properties of the produced compounds (H1 to H12). We tested the oxazepine compounds (H7, H8, H9, H10, H11, and H12) against different types of bacteria, such as gram-positive (Staphylococcus aureus) and gram-negative (E. coli), to see how well they worked against bacteria compared to common antibiotics like ciprofloxacin and norfloxacin. We employed three concentrations in DMSO: (0.01, 0.001, and 0.0001) mg/ml. The compounds  They exhibited the most potent antibacterial activity against Staphylococcus aureus and Escherichia coli. This implies their potential for use as antimicrobials.