Synthesis, Characterization, and Subacute Toxicity of Hydrazide-Iron Complex in Rats

In this study, a hydrazide derivative and its iron (III) complex were synthesized and characterized using IR, MS, NMR, and elemental analysis. Subacute oral toxicity was assessed in male Wistar rats over 28 days. Biochemical and hematological parameters indicated dose-dependent effects on the liver and kidneys, including elevated ALT and creatinine levels. The iron complex group showed reduced RBC and hemoglobin with mild leukocytosis. These results suggest potential systemic toxicity at higher doses, highlighting the need for further investigation.