Preparation and Characterization of New Imidazole Derivatives Derived From Hydrazones and Study of their Biological and Laser Efficacy

This research aims to synthesize and characterize imidazole derivatives from the reaction of hydrazones with amino acids (glycine and alanine) using conventional methods. The validity of the synthesized compounds was confirmed through physical measurements such as melting point and color, as well as spectroscopic methods such as infrared spectroscopy and proton nuclear magnetic resonance spectroscopy. The research methods included testing the biological effectiveness of the compounds against two types of bacteria: Gram-positive Staphylococcus epidermidis and Gram-negative Klebsiella pneumoniae. The compounds (M11-M15) were also irradiated using a neodymium nanosecond laser system (Nd laser) with a wavelength of 808 nm and a frequency of 5 Hz. Changes in melting point and color were measured after irradiation. The results showed that the synthesized compounds exhibited significant antibacterial activity against both types of bacteria. Additionally, some compounds demonstrated high stability under laser irradiation, while others experienced changes in melting point and color, indicating alterations in bond structures.