Publication Details
Abstract
The current study aims to quantify the amounts of inflammatory cytokines exposed to different lipopolysaccharide concentrations isolated from Salmonella typhi. Using the chloroform-methanol procedure, LPS was isolated from S. typhi and then dried with a lyophilizer device. The purity of LPS was confirmed by gas chromatography-mass spectrometry (GC-MS). Doses of 0.625, 1.25, and 2.5 mg/100 g body weight (B.W) were used in this experiment. Four groups of white mice were injected with three doses of LPS, with each group receiving three replicates, aged (2–3) months injected intraperitoneally (IP) with three doses of LPS every 48 hours, and followed by a final booster dose one week later. Subsequently, blood samples were collected and serum levels of interleukin-1 (IL-1) and interleukin-7 (IL-7) were measured by enzyme-linked immunosorbent assay (ELISA) method. The results showed that IL-1 levels were (9.933 ± 0.513 , 9.033 ± 0.568 and 10.700 ± 0.101) pg/ml at the concentrations of 0.625, 1.25, 2.5) mg/100 g (b.w) respectively, compared to the control sample which was (5.933 ± 0.890). Furthermore, the levels of IL-7 were 304.000 ± 0.208, 301.866 ± 0.276 and 333.566 ± 0,164) pg/ml at 0.625, 1.25, and 2.5 mg/100 g (b.w) respectively, while the control sample was (290.700 ± 0.112) pg/ml. The gain of lipopolysaccharide from S. Typhi provoked the immune system inside the injected rats, leading to the secretion of cytokines like IL-1 and IL-7 in any respect tiers used in comparison to the manage. In addition, the lifestyles of IL-1 could limit the manufacturing of IL-7.