Synthesis, Characterization, and Validation Of Novel Thiazine Derivatives For Their Biological And Laser Activity

The research involves the use of chalcones as intermediates, the synthesis of new hexagonal rings from thiazine precursors by reacting previously prepared chalcones with thiourea, and minor modifications to the usual working methods to show that they generate these new rings. The activity of the prepared compounds was proven by physical and spectroscopic techniques such as FT-IR and (1H, 13C-NMR) spectroscopy, as well as the determination of melting point and purity and monitoring the progress of the reaction by thin layer chromatography. The TLC was evaluated using two bacterial isolates known to be resistant to antibiotics, namely Gram-negative Escherichia coli (Gram-ve) and Gram-positive Staphylococcus aureus (Gram+ve). They were compared with the antibiotic amoxicillin as a control agent, and the results showed good inhibitory activity against both types of bacteria used, with high efficacy and selectivity. A helium-neon laser (visible laser) was used to evaluate the laser efficiency of some of the synthesized compounds. Each compound was irradiated for four different periods (15, 30, 45, and 60 seconds) and then physically irradiated. The compounds were examined again, and the surface changes of the compounds" were observed.