Publication Details
Abstract
The study included the isolation and identificstion of some species of pathogenic bacteria from wounds and in burn patients in Kirkuk city during the period from January 2023 to March 2024. 100 samples were collected from in Azadi Teaching Hospital in Kirkuk City The isolates were diagnosed by routine methods, and the diagnosis was confirmed by using the Vitik Compact system. The percentage of wound samples was 55% and the percentage of burn samples was 45%. The results of the culture showed that 15 isolates of Staphylococcus aureus appeared at a rate of 23% and 13 isolates of Escherichia coli at a rate of 20%. The highest bacterial infection rate was in the age groups (60-75) years with 25 isolates 25% and the age group (1-30) years recorded the least infection with 17 17.5%, while the infection rate of males was higher than females at 37% and 27% for females. The effectiveness of the manufactured nanoparticles was tested against bacterial isolates, as five different concentrations were used: 3, 6, 12, 25 and 50 micrograms per ml. All concentrations showed inhibiting the growth of most bacterial isolates. The results showed that silver nanoparticles recorded the highest inhibitory activity at a concentration of 50 micrograms on E.coli and Staph. Aureus with inhibition diameters (19 and 13) mm, respectively. The results Showed the synergism between the particles and ampicillin against E.coli and S.aureus bacteria with an inhibition diameter of 30 and 36 mm, respectively.