Publication Details
Abstract
The study was conducted on 30 three-month-old bisexual
white laboratory outbred rats with an average weight of 80–120 g. The rats were kept in vivarium
conditions. The experimental model was close to human childhood, for which purpose white rats
aged 14–18 days and weighing 16–20 g were introduced into the experiment. Arthritis modeling
was performed as follows: after 2 weeks of the experimental animal’s life, under the skin of the
rat’s hind paw (subplantar) For 1 month, Freund's complete adjuvant (0.01 ml), which contained
0.01 mg BCG, was administered 2 times a week. The autoimmune process was modeled by
sensitizing the animal's body for 28–30 days. The animals whose adrenal tissue was supposed to
be studied were divided into 2 groups of 15 rats (n = 15): group 1 – healthy animals (control
group); The 2nd group consisted of animals with a model of adjuvant arthritis that did not receive
treatment. 10 days after the end of the experiment, the animals were removed from it under ether
anesthesia [12]. The work was carried out in accordance with the rules approved by the Ethics
Committee of the Bukhara State Institute of Medicine, based on the principles of humanism in
relation to animals, provided for by the “Rules for carrying out work with experimental animals”
under the name of Abu Ali ibn Sino.