Antibacterial Efficacy of Ammonium Nanoparticles Synthesized from Ananas Comosus Fruit Extracts Against Streptococcus Sanguis and Enterococcus Faecalis (An in Vitro Study)

the study aimed to biosynthesize NH nanoparticles (NH Nps ) from Ananas comosus (pineapple fruit) extracts and estimate their antibacterial efficacy against Streptococcus sanguis and Enterococcus faecalis.
Material and methods: The aqueous and ethanolic extracts were prepared from the core and pomace tissues of pineapple fruit separately and the NH Nps were synthesized by the addition of 5ml of 1mMolar of an aqueous solution of ammonium sulfate then we notice color change from white to light pink, The EDX, UV Spectrophotometer, FTIR and SEM were used to characterize NH Nps, turbidity method was carried out for the antibacterial activity and the absorbance of 1/1,1/2 and 1/4 dilutions of the four extracts against oral bacteria[ Enterococcus faecalis and Streptococcus sanguis ] was measured at 530 nm by vis-spectrophotometer.
Results: NH Nps were synthesized and characterized, and all the prepared extracts and the nanoparticles had an antibacterial effect against Enterococcus faecalis and Streptococcus sanguis, the best antibacterial effects of NH Nps biosynthesized from aqueous core extract (0.172) , (0.234) for ethanolic core extract,(0.251) for ethanolic pomace extract and ( 0.252) for aqueous pomace extract against Enterococcus faecalis , (0.201) aqueous extract of core,( 0.301) aqueous extract of pomace,( 0.601) ethanolic extract of core and (0.610) ethanolic extract of pomace against Streptococcus sanguis.




Conclusion: The results showed the bactericidal effect of the aqueous extract of pineapple more than the ethanolic extract on Enterococcus faecalis and Streptococcus sanguis, as well as the sensitivity of Enterococcus faecalis and Streptococcus sanguis to biologically synthesized nanoparticles (NH NPs) made from the aqueous and ethanolic extracts.