Publication Details
Abstract
In this work, Fusarium solani (an endophytic fungus) was isolated from Coriandrum sativum L. healthy leaves. Its molecular identification was based on the ITS1 gene. Effect of endophytic fungal extract on a few pathogenic fungi and bacteria found at Tikrit University. In the current study, Fusarium solani was isolated from C. sativum leaves and showed 99% identity with genes in NCBI. Some secondary metabolites were detected in the extracts of F. solani and C. sativum which included total alkaloids, saponins, terpenoids, flavonoids (rutin 100 mg), and phenolic compounds (gallic acid 100mg). F. solani showed high total phenolic compounds (179.87 mg/ml) and showed significant activity against 29 isolated fungi belonging to Candida albicans, C.tropicalus, C.parapsilosis, C.krusei, C.kefyr, C.glabrata, and C.dubliniensis. The study showed the highest inhibition diameter of fungal isolation of C.kefyr was 39 mm at a concentration of 40 mg. ml and the lowest inhibition diameter was recorded in two fungi C.glabrata and C.dubliniensis with a diameter of 10.7 mm and a concentration of 20mg. ml. also the result showed that F. solani extract given high activity against 14 isolates of bacteria, the highest inhibition diameter of bacteria isolation of E.faecalis was 39.4 mm at a concentration of 40mg. ml and the lowest inhibition diameter was recorded in K.pneumoniae with a diameter of 10 mm and a concentration of 20mg. ml.