Publication Details
Abstract
Introduction: Chronic acne vulgaris (AV) is a skin inflammatory condition that affects the pilosebaceous glands. Aim: Isolation and identification of Staphylococcus epidermidis from acne vulgaris in Dhi Qar city and study of some genes (tetK, hlb and nuc) of Staphylococcus epidermidis. Materials & methods: 210 specimens were collected (113 females and 97 male) from acne patients with age ranged from (14-38) years old. From August to September of 2022, a dermatologist from Thi-Qar province in Iraq used a private clinical lab to make a clinical diagnosis for the patients. Specimen were taken from the forehead, cheek, and chin of the patients. 70% ethanol was used to sanitize the sampling site. Transport media was employed to collect the samples. A lancet was used to make a scrape on the lesion's surface, and the fluid was then removed with light hand pressure in order to remove and extract the closed comedones and the papules. Results: It was discovered that 87 (41.4%) of the total samples showed positive results for the growth of S. epidermidis in the best cultured media, including blood agar, mannitol agar, and MacConkey agar. Out of all the samples, 123 (58.6%) showed negative results for S. epidermidis growth. Staphylococcus epidermidis showed total resistance to Penicillin, Oxacillin and Cefoxitin and total sensitive to Nitrofurantoin. The results of PCR assay revealed that 75 (86.2%) of S. epidermidis isolates giving positive results for tetK gene. Despite the fact the hlb gene finds in percentage 21 (24.1%), while 42 (48.3%) of S. epidermidis harbored nuc gene, as represented in Table (4-6). The whole genomic DNA of S. epidermidis isolates.