Publication Details
Abstract
Thirty random cases of Iraqi families contain data of father/son DNA haplotypes have been analyzed between 2017-2022 to find out the usefulness of DYS 391 Locus in paternity testing using power-plex ®fusion and power-plex ®Y23systems. Scientifically, all cases were confirmed and officially reported as an absolute cases of exclusion paternity. A combination of the two systems should permit effective analysis with sufficient diversity and discrimination power of the locus. The results show significant match (i.e., inclusion) at the DYS391 locus between alleged fathers and corresponding sons for both kits in all the cases meanwhile there were no match (i.e., exclusion) in many autosomal DNA loci and the rest Y-haplotypes other than DYS391. Furthermore, the results revealed only three DYS391 allele polymorphisms; Allele number 10,11and 9 in a percentage of 69.23%, 27.692% and 3.076% contained in all 30 cases respectively. Allele frequencies, homozygosity, haplotype diversity (HD), match probability and the power of discrimination of DYS391 locus (PD) were estimated. The observed allele frequencies were (0.692, 0.276 and 0.030) for allele numbers 10, 11 and 9 respectively. Homozygosity was found to be (0.478, 0.0761 and 0.0009) respectively too. Measurement of the upper bound confidence interval (CI) for each corresponding allele was (2.642, 2.234 and 1.989). Haplotype diversity was (0.451) and combined power of discrimination for DYS391 Locus was (0.4442). As a conclusion, there is a limitation in certifying DYS391 locus as an exclusive locus useful Y-STR in testing paternity and male lineage in autosomal STR kits because of little allele variants, poor power of discrimination and gene diversity.