Using Safranine Dye for Indirect Spectrophotometric Determination of Furosemide in the Presence N-bromosuccinamide

A sensitive and accurate indirect spectroscopic method has been proposed for the determination of furosemide in acidic media. The method includes oxidation of safranin dye in the presence of N-bromosuccinimide as an oxidizing agent to form a pink-colored product that can be measured spectrophotometrically at a wavelength of 526 nm. The molar absorbance and Sandel sensitivity were (2.4×104 L/mol.cm) and (0.0135 µg\cm) respectively, the correlation coefficient was 0.9995 with a recovery rate of 97.24%, a standard deviation of 0.867%, 0.0966 µg\ml for (LOD) and 0.322 µg\ml for (LOQ). The technique was applied to determine the purity of furosemide and the medicinal forms of it.