Publication Details
Abstract
Colorectal cancer continues among most serious life-threatening tumors worldwide. Recently, as a result of the progress made in microbial science and its relationship to cancer, and the efforts made to eliminate this dangerous disease without affecting normal cells, the current study aimed to shed light on Bacteria-mediated cancer therapy as a promising therapeutic tool with less side effects and due to its high immune stimulation effect. Colon cancer was induced in male albino rats using Azoxymethane (AOM) at 15mg/Kg bw, the curative effect (post-treatment) of Salmonella typhimurium ghosts (STGs), which was prepared using chemical concentrations selected in a special protocol, was studied by inoculated the animal subcutaneously with a 100 μl/rat in single dose with equal volumes (1:1) of STGs +Freund's adjuvant. We vaccinated the animal with (STGs) in 3 cycles. After 42 days of the whole vaccination, the animals were sacrificed, the colorectal tissues were removed and preserved in a diluted formalin solution until performing grossly and histological examinations. The result revealed that histolpathological changes in induced colorectal cancer includes damage and alterations in mucosal crypts architecture, increase spaces between crypts, dysplastic changes in crypts epithelium. In vaccine treated animal showing severe histological alterations, infiltration of inflammatory cells and significant hyperplasia in lymphoid tissue, comparing with the control group. In conclusion, vaccination by STGs vaccine using S. typhimurium will enhance the immune response, which is a promising medication for the treatment of colon cancer.