In Vivo Evaluation of Pumpkin Seed Extract on Entamoeba histolytica Activity

In regions like Iraq, where amoebiasis is prevalent, finding effective treatments against Entamoeba histolytica is crucial due to the emergence of drug-resistant strains. This study investigates the potential antiamebic properties of the ethanolic extract of pumpkin seeds (Cucurbita pepo) as an alternative medication. Using in vitro and in vivo assays, the efficacy of C. pepo extracts against E. histolytica was evaluated, with metronidazole as a comparative control. GC-MS analysis identified compounds with known antimicrobial and anti-inflammatory properties in the extract. Results showed significant anti-amoebic activity of C. pepo extracts, particularly at a concentration of 500 µg/ml, with mortality rates ranging from 58.13% to 86.95%. Additionally, previous studies corroborate the anthelmintic properties of C. pepo against various parasites. These findings suggest the potential of C. pepo as a natural treatment for amoebic dysentery, highlighting its broader implications in combating parasitic infections and addressing the limitations of current therapies.