Publication Details
Abstract
Saccharomyces cerevisiae, a widely used and safe yeast in biotechnology, was evaluated for its ability to produce an environmentally friendly biosurfactant using various carbon sources. This study aimed to compare biosurfactant production from glucose, crude oil, olive oil, and cooking oil, addressing the gap in understanding the impact of insoluble carbon sources. Yeast was activated in YEPG broth and cultured in modified YEP medium with different carbon sources. The biosurfactant activity was assessed through surface tension reduction (ST) and oil spreading method (OSM). Results indicated that glucose did not significantly enhance biosurfactant production (60mN/m ST, 3mm OSM), while crude oil (50mN/m ST, 45mm OSM), olive oil (53mN/m ST, 40mm OSM), and cooking oil (54mN/m ST, 35mm OSM) significantly improved activity. The findings demonstrate that insoluble carbon sources are crucial for effective biosurfactant production by S. cerevisiae, with crude oil yielding the most active biosurfactant. This research highlights the potential of using oils to enhance biosurfactant production, providing insights for industrial applications.