Publication Details
Abstract
Staphylococcus aureus and Pseudomonas aeruginosa are the leading causes of urinary tract infections. These bacteria have also become resistant to antibiotics, and accordingly, attention has been directed towards medicinal plants as alternative treatments. Objective: To study the inhibitory effect of alcoholic and aqueous extract of Ziziphus spina-christi against pathogenic bacteria. In this study, 100 samples were collected from urine from patients attending and lying in a hospital (Mosul Teaching Hospital - Public Health Laboratory) of various ages and genders for the period from December 1, 202 3 , to March 10, 2024, Preparation different concentrations (25, 50, 75 and 100 ) mg/ml of an alcoholic and aqueous extract of the Sidr plant. The results indicated that the number of isolates that showed positive growth was 57 out of 100 isolates distributed between S. aureus and P.aeruginosa. In contrast, the rest of the isolates showed negative growth. Laboratory tests were conducted on 26 of 50 samples positive for P. aeruginosa. The Gram-positive isolates included 31 isolates, with a percentage of (50%), S. aureus, out of a total of 50 isolates, and the Gram-negative isolates, with a rate of (50%), P.aeroginosia, out of a total of 37 isolates. At (25, 50, 75 and 100 ) mg/ml, the alcoholic extract recorded inhibition zone was (5.5, 10.12, 12.7, 15.55)mm and (6.77, 12.90, 17.77, 20.1) mm against P.aeruginosa and S.aureus respectively, while aqueous extract recorded inhibition zone (1.01, 2.22, 3.05, and 3.75) mm, against S.aureus. It was also observed that the cold aqueous extract had no inhibitory effect on P.aeruginosa isolates. Alcoholic extracts were more effective against both bacteria types than aqueous extracts for all concentrations.