Study the Antibacterial activity and Immunomodulatory Effect of Aqueous Commiphora myrrh Leaves Extract in vivo

Commiphora myrrh demonstrated antibacterial efficacy against microorganisms.  Myrrh stimulates the activation/maturation and differentiation of both (myeloid and lymphoid) cell types of WBCs during wound healing of caused stomach and skin injuries, and it has significant anti-inflammatory, analgesic, and anti-hyperlipidemia efficiency. The aim of this study is to investigate the antibacterial activity  and immunomodulatory  effect of aqueous  C. myrrha  extract. Pseudomonas aeruginosa was collected from the Microbiology Laboratory/College of Health and Medical Technology/Sawa University. C myrrh leaves were bought from the Iraqi market and dried for extraction. Concentrations of 25, 50, 75, and 100 mg/ml were created. The experimental design included two parts, in vitro and in vivo. The in vitro investigation estimated the antibacterial activity using the agar diffusion method and  in vivo study was  Included four groups. Group I (Control) received daily intraperitoneal injections of normal saline. Group II received intraperitoneal injections of 1.5 x 108 CFU/ml of P. aeruginosa. Group III received oral administration of 100 mg/ml extract. Group IV received intraperitoneal injections of 1.5 x 108 CFU/ml of P. aeruginosa followed by oral administration of 100 mg/ml extract for 14 days. then notice the clinical signs of each group followed by estimation , TLR-2 , IL-17 , IL 10 , arthus reaction and delayed hypersensitivity. The extract demonstrated strong antibacterial activity against the tested isolates. The maximum activity was seen at a dose of 100 mg/ml, producing an inhibition zone of 1.6 ± 0.01. The animals in group IV had the highest TLR-2, IL-17, and IL-10 levels with a significant difference (p≤ 0.05).The arthus test and DHT results showed that the group that received a mixture of extract and P. aeurginosa had significantly greater values than the other group (p < 0.05). C. myrrh aqueous extract leaves  has immunomodulatory  effect on the immune response ,innate  immunity and acquired immunity  as well as it showed the antibacterial activity in vitro .