Publication Details
Abstract
Objective: Avulsion injuries often require advanced reconstructive approaches such as pedicle skin grafting, yet graft survival and healing remain challenging. This study aimed to investigate the clinical and biochemical effects of autologous platelet-rich fibrin (PRF) on pedicle skin graft healing in goats Materials and method: Fifteen healthy female goats (7–8 months old, 18–20 kg) were subjected to bilateral standardized trunk wounds. Pedicle skin flaps (2×6 cm) were advanced over 2×2 cm defects and divided into two groups: control (without PRF) and PRF-treated. PRF was prepared by centrifugation of autologous blood samples and applied locally before wound closure. Clinical assessments (exudation, swelling, and graft appearance) were conducted postoperatively. Biochemical analysis included hydroxyproline content (days 7, 14, and 21) and wound surface pH (days 5, 7, 14, and 21). Data were analyzed using independent samples t-test (p ≤ 0.05) Results: The PRF-treated group demonstrated a significantly faster resolution of swelling and higher exudation scores within the inflammatory phase, consistent with accelerated healing. Hydroxyproline levels were significantly elevated in PRF-treated grafts at days 7 and 14, indicating enhanced collagen synthesis, but returned to baseline by day 21. pH analysis revealed significantly higher alkalinity in PRF-treated wounds compared with controls, suggesting improved wound microenvironment Conclusions: Autologous PRF accelerated pedicle graft healing in goats by enhancing collagen deposition and optimizing wound pH. These findings highlight the potential of PRF as an effective biological adjunct in reconstructive surgery