Publication Details
Abstract
Purpose: Researchers in this study set out to determine if periodontal disease patients' saliva had any histological alterations or oxidative stress. Materials and Methods: In our study, we compared two groups of periodontitis patients, one with stage I and another with stage II, using saliva and serum samples. We also included a group of 15 volunteers who had no dental restorations. We used the thiobarbituric acid technique to dose malondialdehyde MDA and Ravin's method to determine ceruloplasmin. The periodontal fragments were prepared for histopathological analysis by quickly fixing them in 10% neutral formalin. Then, they were embedded in paraffin according to the standard histology protocol. Results: Patients in the second stage of periodontitis had lower salivary MDA readings than those in the first stage. Compared with the control group, patients with stage I periodontitis had a much higher blood concentration of MDA; however, in individuals with stage II periodontitis, this concentration was much lower. In the control group, there are no statistically significant differences in serum ceruloplasmin levels between patients with periodontitis stage II and those with periodontitis stage I. Conclusions: Since there is a greater concentration of malondialdehyde (MDA) in the circulation than in saliva, it cannot be a byproduct of blood filtering. There is a robust relationship between biochemical factors and tissue changes. Plaque removal from teeth helps keep mouths healthy.