Detection of Molecular Typing of the Intestinal Parasite E. histolytica in Humans

This study was conducted on patients visiting Tikrit Teaching Hospital and some private laboratories in Tikrit from the beginning of January 2023 to the end of December 2023. A total of 220 stool samples were collected, with a percentage of 22.72% positive for Entamoeba histolytica, based on 50 positive microscopic tests. The results demonstrated the success of the Polymerase Chain Reaction (PCR) technique in amplifying the gene responsible for encoding the Galactose/N-acetyl-D-galactosamine lectin enzyme from E. histolytica samples indicating the method's effectiveness and efficiency in diagnosing the targeted genetic sequence.