Detail Publikasi
Abstrak
Folate, forms of which are known as folic acid and vitamin B9, is one of the B vitamins 2 Folic acid helps your body produce and maintain new cells, and also helps prevent changes to DNA that may lead to cancer therefor our study describe sensitive, precise, and dependable spectrophotometric technique has been developed to accurately detect the concentration of vitamin B9 Folic acid in pure and pharmaceutical formulations. Depend on formation of ion-pair between vitamin B9 and methyl violet 10B dye. This reaction results in an orange-coloured solution that is soluble in water and shows absorption maxima at wavelengths of 446 and 367 nm when compared to a blank solution. The analytical technique was implemented and validated by thoroughly examining and optimizing various factors that could potentially disrupt the reaction. Significant linear relationships, characterized by correlation coefficients ranging from 0.9968 to 0.9974, were observed under optimal conditions. These associations remained consistent throughout concentration ranges of 0.625- 12.5μg/ml for 446 ,367 nm respectively. While the value of the molar absorption coefficient 48907.12 & 40873.64 L.mol-1.cm-1 at 446,367 nm respectively with the limits of qualitative and quantitative detection 0.1624,0.5415 and 0.1619 , 0.5399 μg / ml , respectively.The proposed method was successfully applied for the determination of folic acid in its pharmaceutical preparations, from Erbil Company – Iraq. Trade name"(folic acide).