Mebeverine Hydrochloride in Pharmaceutical Preparation as Determined by Spectrophotometer Using the Ion Association Reaction

This study aims to develop a simple, accurate, and sensitive colorimetric method for determining Mebeverine Hydrochloride (MBV) in its pure form and pharmaceutical preparations. The method relies on the formation of an ion-association complex between Mebeverine and picric acid, with maximum absorption measured at 382 nm. Using spectrophotometry, the method demonstrates high sensitivity, with a molar absorptivity of 3.41×10⁴ L mol⁻¹ cm⁻¹ and a linear calibration range of 1–30 µg/ml (R² = 0.990). The experimental procedure optimizes factors such as picric acid concentration, reaction time, and solvent selection to enhance accuracy and reproducibility. Validation of the method shows high precision (RSD: 0.4076–2.949%) and recovery rates (94.85–99.31%). Interference studies confirm that common excipients do not affect the analysis. The results indicate that the proposed method is effective for routine quality control of MBV in pharmaceutical formulations, offering simplicity, stability, and reliability in drug quantification.