Determination of vitamin D3 in biological fluids by Sophonix Device that operates on chemiluminescent immunoassay (cLIA)

Vitamin D deficiency, affecting over a billion people globally, is now recognized as a steroid hormone crucial for immunity and bone health. Hashimoto’s thyroiditis, an autoimmune thyroid condition, is linked to low vitamin D. This study compared vitamin D and calcium levels in 105 individuals from Baghdad (2023): healthy controls, hypothyroidism, and hyperthyroidism patients.  Results revealed a significant decrease in vitamin D levels in the hyperthyroidism group compared to the healthy group (p-value = 0.008), while the hypothyroidism group showed no statistically significant difference (p-value = 0.056). Calcium levels were significantly reduced in hypothyroidism patients (p-value = 0.000) and moderately reduced in hyperthyroidism (p-value = 0.000).Hyperthyroidism was characterized by low serum TSH levels alongside elevated T4 and T3, whereas hypothyroidism showed the highest TSH levels and reduced T4. These findings highlight a clear association between thyroid disorders and imbalances in vitamin D and calcium, emphasizing the need to monitor these parameters in diagnosis and management.